Instructions for preparing the Autovaccine

Instructions for preparing the Autovaccine

Lege artis
Protocol published in Beltrum, June 2021

The following protocol has been made to explain how to prepare the Autovaccine for use in the Autovaccine TherapyTM as conceived and developed by doctor L Kunst (MD).  

The environment for the preparation process should be hygienically clean and the preparation method must strictly comply with this protocol.




NOTE: Should the practitioner have access to an incubator, it is recommended to warm all the requirements to 37oC before commencing with the removal of blood from the patient. The period of gentle rocking should also take place in the incubator. This is not essential, but it appears to improve the effectiveness of the reaction and is more comfortable for the patient.
You can see a video instruction movie over here

List of requirements.

Personal Protection Equipment

  • Sterile medical gloves
  • Mask and/or perspex face protection
  • White coat
  • Hygienic rubbish containers for needles, syringes, vacutainers, paper and plastic

For removing 3 ml blood from the patient

  • Safety Lock blood collection set + Luer adapter 21G
  • (vacutainer system + heparinized)
  • 1 x vacutainer Sodium heparin 10 ml green cap;
  • Tourniquet, cotton buds, iodine, plasters

For preparing the Autovaccine

  • 1 x bottle of iodine in alcohol (for sterilizing) or hospital grade alcohol.
  • 1x ampule 10 ml of Fresenius sterile water for injection
  • 1x sterile ampule of Fresenius natrium chloride 10% solution
  • 2 x 10 ml Luer lock syringes 
  • 1x 1 ml tuberculine Luer lock syringe (or similar)
  • 1x yellow code injection needle – for autovaccine preparation (0,9x25mm)
  • 1x purple code injection needle – for injection into the patient (0,55 x 25mm)
  • 1x female/female connector for 2 syringes
  • 2 x three-way red Discofix taps or 2 sterile caps to be placed on the water and the saline solution containers for when more than one patient is treated that day.
  • Stainless steel artery clamp to hygienically mount the connectors onto the syringes.

Rocker machine (e.g. Edmund Bühler) to gently shake the autovaccine preparation.


  • Incubator (strongly recommended)


    1. Use the 10 ml luer lock syringe to remove 3 ml of sterile water for injection from the 10 ml container (no needle required). Disconnect the syringe and attach the female/female connector – leaving it partly inside the packaging for safety and place in an incubator at 37oC (if available). Otherwise just place carefully on the work surface.
    2. Using the normal procedure for taking a venous blood sample, remove 3 ml of blood using a heparinized vacutainer. 
    3. Take the second 10 ml luer lock syringe and attach the yellow code needle (0,9 x 25 mm). Use this to remove 2 ml of blood from one of the vacutainers. Leave the needle in the vacutainer cap and use the artery clamp to disconnect the needle from the syringe. Dispose of the vacutainer with the remaining blood and the needle.
    4. Connect the syringe with 2 ml blood to the syringe with water using the female/female connector. Go not mix yet. If you have an incubator, at this stage you should put the coupled syringes in the incubator at 37oC for 10 minutes.
    5. Take the connected syringes and mix the two components by alternately pushing the plunger from one syringe into the other and then back again. Pump 12 times each way as is shown in total 24 times. Leaving the total fluid of 5 ml in one syringe at the end.
    6. Disconnect the empty syringe from the connector temporarily. Draw in the plunger of the full syringe to fill up the remaining volume of the syringe containing the blood mixture with air. Reconnect the empty syringe.
    7. Place the syringes still connected to each other on the rocker apparatus (in the incubator – if available) and rock gently for 1 hour and 15 minutes.
    8. Meanwhile, take the 1 ml luer lock syringe and remove 0.3 ml of sterile saline solution from the Fresenius container. Leave the syringe attached to the container until ready for use (in the incubator if available).
    9. Once the 1 hour and 15 minutes is elapsed, remove the connected 10 ml syringes from the rocker. Disconnect the empty 10 ml syringe from the full one leaving the connector on the syringe with blood. Take the 1 ml luer lock syringe with saline and disconnect from the container. Fix the 1 ml luer lock syringe with saline to the blood syringe via the connector. Mix the two syringes thoroughly as in step 5. Twelve times each way, 24 pumps in total – leaving all the blood mixture at the end in the 10 ml syringe. At this stage the air should be removed from the syringe. This is now the autovaccine.
    10. Disconnect the female/female connector with the 1 ml luer lock syringe from the syringe with the blood mixture and attach instead the purple injection needle (0,55 x 25 mm).
    11. The autovaccine preparation can now be subcutaneously injected into the patient’s abdomen. (see notes below)
    12. The autovaccine is NOT designed to be stored. 


Essential notes for future treatments

NOTE 1: Depending on the reaction of the patient to his or her own personal vaccine, the volumes of the ingredients can be increased or decreased. For example, it can be made stronger to induce more reaction by increasing the water to 4 ml and the blood to 3 ml and the 10% saline solution to 0.4 ml. The ratio can go one step higher if necessary, to 5 ml water + 4 ml blood + 10% NaCl 0.5 ml. For this volume of fluid, it is recommended to use larger syringes for a more effective pumping action.

NOTE 2: the volume of water must always be higher than the volume of blood.

NOTE 3: the ratio water to saline must be 90% : 10% to make it an isotonic solution. 

The formula is H2O : NaCl = 1 : 0.1 

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